4/16/2023 0 Comments Geneious and geneious prime![]() Our results showed complex interactions among these viruses. Here, we studied the effect of STV in co- and triple-infections with Cucumber mosaic virus (CMV) and Pepino mosaic virus (PepMV). Accordingly, the reported symptoms could be induced by the interaction of STV with other viruses, which frequently infect tomato. Subsequent studies showed that the virus did not induce apparent symptoms in single infections. Southern tomato virus (STV) is a persistent virus that was, at the beginning, associated with some tomato fruit disorders. These findings are an important first step toward future investigations to determine if these viruses engender positive effects in common bean, a vital crop in east Africa. In addition, phylogenetic analyses based on sequences generated from the helicase domains showed that geographical distribution does not correlate to genetic relatedness or the occurrence of endornaviruses. Intra- and inter-species sequence comparisons of these genomes with comparator sequences from GenBank revealed clear species demarcation. ![]() Using high throughput sequencing of two east African bean varieties (KK022 and KK072), we confirmed the presence of these viruses and generated their genomes. Notably, this was the first identification of PvEV3 in common bean from Africa. ![]() ![]() Out of 26 bean varieties examined, four were infected with PvEV1, three were infected with both PvEV1 and PvEV2 and three had infections of all three (PvEV) 1, 2 and 3. Using reverse transcription coupled polymerase chain reactions, we investigated if common bean ( Phaseolus vulgaris L.) varieties popular in east Africa were hosts for Phaseolus vulgaris endornavirus (PvEV) 1, 2 or 3. It is speculated that these RNA viruses may be mutualists that enhance plant resilience to biotic and abiotic stresses. Persistent viruses include members of the family Endornavirus that cause no apparent disease and are transmitted exclusively via seed or pollen. ![]()
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